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Cat. No.: TPM-5 (for 5 x 1ml, without dye)

Cat. No.: TPMD-5 (for 5 x 1ml, with dye)

Cat. No.: TPM-20 (for 20 x 1ml, without dye)

Cat. No.: TPMD-20 (for 20 x 1ml, with dye)

Description

The 2× Taq PCR MasterMix is an optimized ready-to-use PCR mastermix, providing several advantages such as rapidity and simplicity, high sensitivity and specificity, and good stability. This mastermix contains all the necessary components for PCR, excluding the DNA template and primers.

Taq DNA Polymerase, included in the 2× Taq PCR MasterMix, lacks 3'→5' exonuclease activity, making it ideal for amplifying DNA fragments that are less than 6 kb in length. Moreover, the resulting PCR product possesses an "A" base at the 3' terminal, making it extremely convenient for cloning into T-vectors.

Our 2× Taq PCR MasterMix also incorporates high-performance PCR enhancers and stabilizers, suitable for both standard PCR reactions and more complex templates such as those with high GC content or secondary structures. The utilization of various PCR enhancers and stabilizers has enabled the development of an efficient and stable pre-prepared PCR mix. This not only simplifies operational procedures, reduces contamination, significantly decreases labor intensity and sampling errors, but also enhances the specificity of PCR reactions. Additionally, the product design relaxes the requirements for PCR conditions, allowing for the amplification of templates with as few as 2 copies, resulting in more precise experimental outcomes.

Our 2× Taq PCR MasterMix is available in two formulations: with or without dye. The dye-containing PCR MasterMix allows for direct electrophoresis after PCR without the need to add loading buffer. This feature further improves the efficiency and convenience of experiments.

Features

• With 5'→3' DNA polymerase activity, 5'→3' exonuclease activity, and no 3'→5' exonuclease activity. 
• The extension speed is 1-2 kb/min.
• Amplify fragments up to 6 kb.
• Allowing for the amplification of templates with as few as 2 copies
• The amplified PCR product has an A-tail, allowing direct TA cloning.
• With exceptional thermal stability, the half-life of the polymerase exceeds 40 min at 95°C.
• Available in two formulations: with or without dye

Component

5 × 1ml of 2 × Taq PCR MasterMix is supplied, which is suitable for 200 runs for 50µl PCR reaction and 500 runs for 20µl PCR reaction.

Quality Assurance

• No exogenous nuclease activity
• No host residual DNA
• No significant change in activity after storing at room temperature for one week

The difference between batches of this product is very small, so it is especially suitable for the following applications:

• Large-scale gene detection
• Semi-quantitative PCR experiment
• micro DNA detection

Example

Storage

The minimum shelf life is 1 year at -20°C.

Instruction: Protocol

Related: U-Taq DNA Polymerase

Only for research and not intended for treatment of humans or animals

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