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2 × LongFastHiFi PCR MasterMix
$168.00 - $672.00
$840.00
2 × LongFastHiFi PCR MasterMix is based on engineered high fidelity enzymes with a variety of elongation factors, which greatly improves the amplification length, amplification speed, fidelity, and yield. The mastermix generates blunt-end PCR products because of the 3’ → 5’ exonuclease (proofreading) activity, which shows approximately 55-fold higher fidelity than Taq DNA polymerase. Therefore, 2 × LongFastHiFi PCR MasterMix provides greater efficiency and elongation capabilities than conventional PCR enzymes. This mastermix contains red tracking dye, which can be directly loaded for electrophoresis without adding a loading buffer.
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All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: LFHF-5 (for 5 ml)
Cat. No.: LFHF-25 (for 25 ml)
Features
- Fast amplification speed: The extension rate can reach 3-4 kb/min, which is 6-8 times that of Pfu DNA Polymerase.
- High amplification yield: Generally, the amount of PCR products is 50%-100% higher than that of traditional Pfu DNA Polymerase.
- Excellent fidelity: The fidelity is more than 55 times that of Taq DNA Polymerase.
- Ideal for long sequences: When using complex genomic DNA as a template, the amplification products can be up to 10kb. When using simple genomic, plasmid, and phage DNA as templates, the amplification products can be up to 15kb.
Quality Control
Each batch of 2 × LongFastHiFi PCR MasterMix must pass the amplification detection of the following two sizes of fragments before shipment:
- Hα1AT, 2.6 kb, extension 20 sec/kb
- ß-globin, 6 kb, extension 20 sec/kb.
Storage
The minimum shelf life is 2 years at -20°C and a month at 4°C.
Instruction: Protocol
Related: 2 × LongFast PCR MasterMix, 2 × FastHiFi PCR MasterMix
Only for research and not intended for treatment of humans or animals
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