Description

CRISPR/Cas9 is a powerful gene editing tool for gene knockouts and knock-ins. Traditional methods using plasmid transfection or lentiviral infection may pose risks of gene fragment insertion and immune reactions. Using RNP complexes (riboproteins formed by Cas9 and sgRNA) as a substitute for foreign vectors, and introducing them directly into host cells via methods such as liposomes, electroporation, microinjection, or cell-penetrating peptides, offers advantages of speed, safety, reduced off-target effects, and higher editing efficiency. This makes it a more efficient approach to implementing CRISPR/Cas9 technology.

SBS Genetech provides HPLC-purified Synthetic Ultrapure sgRNAs with a purity greater than 90%, correct sequencing, and low toxicity. This is used for functional assays of candidate sgRNA in cell models, efficacy and safety evaluations in animal models, and is suitable for the research and verification stages of cell and gene therapy development.

Features

• Enhance Success Rate, Save Time and Labor: Streamlined processes for higher efficiency.
• Purity ≥90%, Correct Sequence: Reduce off-target effects and improve editing efficiency.
• Leading Delivery Time: Accelerate your project with our prompt delivery.
• Customized Production and QC Standards: Precisely match downstream applications at all levels.

Advantages of RNP (Cas9 and sgRNA) Complexes vs. Traditional Plasmid/Lentivirus

• No DNA Interference: Avoids unintended gene fragment insertion.
• No Immune Reaction Interference: Eliminates factors affecting host cells.
• High Transfection Efficiency: Suitable for host cells with low plasmid transfection efficiency.
• Simple and Fast: No need for transcription expression and plasmid degradation, shortening the experimental cycle.
• Improved Editing Efficiency: High binding efficiency and stability of Cas9 protein with sgRNA.
• Reduced Off-Target Effects: Non-persistent expression of Cas9 protein and sgRNA, which can be degraded.

Advantage of sgRNA vs. crRNA: tracrRNA

• Easy to Operate: No annealing operation needed to combine crRNA and tracrRNA.
• More Stable: Tighter binding with Cas9 protein.
• Higher Editing Efficiency: Improves experimental efficiency and success rate, ideal for primary and stem cells.

Related: 

• Synthetic sgRNAs
• Synthetic Ultrapure sgRNAs (HPLC)
• Synthetic Cas12a (cpf1) crRNAs
• Synthetic pegRNA (Prime Editing guide RNA)
• guide RNA Customization Service
• Cas Nuclease (more than 30 types of Cas Nucleases available)

By providing high-quality synthetic sgRNAs and over 30 types of Cas nucleases, SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.

Featured Citations

Interested in seeing published research using our gRNAs?

Orthogonal transcriptional modulation and gene editing using multiple CRISPR/Cas systems

_{Molecular Therapy    |    28 November 2014    |    DOI: https://doi.org/10.1016/j.ymthe.2024.11.024}

_{The sgRNAs for the s. pyogenes Cas9 system were ordered from Synthego as chemically modified sgRNAs containing 2’-O-methyl on the three terminal nucleotides at both ends and  3’phosporothioate between the first three and last two bases. sgRNAs for the s. aureus Cas9 system were synthesized by either Synthego or SBS Genetech Co. with 2’-O-methyl and 3’ phosphorothioate on the three terminal nucleotides at both ends.}

Amplification-Based CRISPR/Cas12a Biosensor Targeting the COX1 Gene for Specific Detection of Porcine DNA

_{ACS Omega    |    8 October 2023    |    DOI: https://doi.org/10.1021/acsomega.3c04473}

_{The designed primers, CRISPR gRNA, and ssDNA probe were synthesized by SBS Genetech Co., Ltd. (Beijing, China). EnGen Lba Cas12a and its corresponding NEBuffer r2.1 were procured from New England Biolabs (Ipswich, Massachusetts, USA), and the RPA Kit used in this study was procured from TwistDx Ltd. (Cambridge, UK). The SYBR Select Master Mix was acquired from Applied Biosystems (Waltham, Massachusetts, USA).}

DDR1 promotes breast tumor growth by suppressing antitumor immunity

_{Oncology Reports    |    27 September 2019    |    DOI: https://doi.org/10.3892/or.2019.7338}

_{Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.}

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory