Description

Prime Editor offers the advantages of no double-strand breaks and no need for a template, making it a safer option for gene knockouts, knock-ins, and mutations. The Prime Editor consists of two parts: Cas9-reverse transcriptase and pegRNA. The pegRNA is composed of a guide sequence (Spacer), scaffold sequence, reverse transcription template (RTT) with the editing site (Edit), and primer binding site (PBS) for reverse transcription. Under the guidance of pegRNA, the Cas9-reverse transcriptase precisely cuts the target DNA single strand and synthesizes DNA with the correct sequence according to the reverse transcription template, completing the gene editing. The length of pegRNA is generally over 110nt. We have extensive experience in chemically synthesizing long-chain RNA, providing products with precise molecular weight and high purity. We support desalting and HPLC purification, over 200 types of modifications, and various customization needs.

Features

• Precise Molecular Weight, High Purity
• Consistent Quality Across Batches
• Editing efficiency can exceed 60%
• Default modifications enhance editing efficiency

Related: 

• Synthetic sgRNAs
• Synthetic Ultrapure sgRNAs (HPLC)
• Synthetic Cas12a (cpf1) crRNAs
• Synthetic pegRNA (Prime Editing guide RNA)
• guide RNA Customization Service
• Cas Nuclease (more than 30 types of Cas Nucleases available)

By providing high-quality synthetic sgRNAs and over 30 types of Cas nucleases, SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.

Featured Citations

Interested in seeing published research using our gRNAs?

Orthogonal transcriptional modulation and gene editing using multiple CRISPR/Cas systems

_{Molecular Therapy    |    28 November 2014    |    DOI: https://doi.org/10.1016/j.ymthe.2024.11.024}

_{The sgRNAs for the s. pyogenes Cas9 system were ordered from Synthego as chemically modified sgRNAs containing 2’-O-methyl on the three terminal nucleotides at both ends and  3’phosporothioate between the first three and last two bases. sgRNAs for the s. aureus Cas9 system were synthesized by either Synthego or SBS Genetech Co. with 2’-O-methyl and 3’ phosphorothioate on the three terminal nucleotides at both ends.}

Amplification-Based CRISPR/Cas12a Biosensor Targeting the COX1 Gene for Specific Detection of Porcine DNA

_{ACS Omega    |    8 October 2023    |    DOI: https://doi.org/10.1021/acsomega.3c04473}

_{The designed primers, CRISPR gRNA, and ssDNA probe were synthesized by SBS Genetech Co., Ltd. (Beijing, China). EnGen Lba Cas12a and its corresponding NEBuffer r2.1 were procured from New England Biolabs (Ipswich, Massachusetts, USA), and the RPA Kit used in this study was procured from TwistDx Ltd. (Cambridge, UK). The SYBR Select Master Mix was acquired from Applied Biosystems (Waltham, Massachusetts, USA).}

DDR1 promotes breast tumor growth by suppressing antitumor immunity

_{Oncology Reports    |    27 September 2019    |    DOI: https://doi.org/10.3892/or.2019.7338}

_{Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.}

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory