Synthetic Cas12a (cpf1) crRNAs
Description
Cas12a (Cpf1) is a novel CRISPR-Cas DNA nuclease, providing a significant complement to the Cas9 system with the following advantages:
Expanded Editing Sites: Cas12a recognizes T-rich PAM sequences, enabling genome editing in AT-rich genomes (e.g., zebrafish).
Enhanced Editing Efficiency: Cas12a creates staggered sticky ends at the 5' end of the sequence, improving homologous recombination repair (HDR) efficiency and thus increasing gene editing efficiency.
Shorter gRNA: Cas12a's crRNA is around 40-44 nucleotides (including a 20-nucleotide constant region and a 20-24 nucleotide specific binding region), making chemical synthesis simpler and more cost-effective.
Leveraging over 20 years of oligonucleotide synthesis experience, SBS Genetech offers customized Cas12a crRNA synthesis services, supporting AsCas12a/LbCas12a and various other customized Cas12a crRNA syntheses. With high purity, precise molecular weight, and verified high gene knockout/knock-in efficiency, it is an ideal choice for conducting Cas12a-related gene editing experiments.
Features
Outstanding Product Quality
Exact molecular weight with high purity
Excellent batch-to-batch consistency, ranging from RUO to GMP standards
Proven Gene Editing Efficiency
Tested and validated across multiple cell lines and Cas12a enzymes
High efficiency in both gene knockouts and knock-ins
Flexible Service Options
Choose between cost-effective (desalting purification) or high-purity (HPLC purification) options
Applications
- Gene Knockout/Knock-in: Suitable for species with AT-rich genomes.
- Gene and Cell Therapy: High editing efficiency with low off-target effects.
- In Vitro Diagnostics: Utilizing DETECTR technology for virus and tumor detection.
Service Details
Note:
- For AsCas12a and LbCas12a, you can directly provide the variable region sequence of 19-29 nt, and we will automatically add the verified scaffold sequence for you. For other Cas12a, you can directly provide the complete 40-49 nt sequence.
- Standard 2'-O-Methylation and thiolation modifications are included at no extra cost. For other customized modifications, please inquire for details.
- Other delivery quantities are available upon request, up to 5000 nmol.
- Various other customized QC options are available upon request.
Related:
- Synthetic sgRNAs
- Synthetic Ultrapure sgRNAs (HPLC)
- Synthetic Cas12a (cpf1) crRNAs
- Synthetic pegRNA (Prime Editing guide RNA)
- guide RNA Customization Service
- Cas Nuclease (more than 30 types of Cas Nucleases available)
By providing high-quality synthetic sgRNAs and over 30 types of Cas nucleases, SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.
Featured Citations
Interested in seeing published research using our gRNAs?
Orthogonal transcriptional modulation and gene editing using multiple CRISPR/Cas systems
Molecular Therapy | 28 November 2014 | DOI: https://doi.org/10.1016/j.ymthe.2024.11.024
The sgRNAs for the s. pyogenes Cas9 system were ordered from Synthego as chemically modified sgRNAs containing 2’-O-methyl on the three terminal nucleotides at both ends and 3’phosporothioate between the first three and last two bases. sgRNAs for the s. aureus Cas9 system were synthesized by either Synthego or SBS Genetech Co. with 2’-O-methyl and 3’ phosphorothioate on the three terminal nucleotides at both ends.
Amplification-Based CRISPR/Cas12a Biosensor Targeting the COX1 Gene for Specific Detection of Porcine DNA
ACS Omega | 8 October 2023 | DOI: https://doi.org/10.1021/acsomega.3c04473
The designed primers, CRISPR gRNA, and ssDNA probe were synthesized by SBS Genetech Co., Ltd. (Beijing, China). EnGen Lba Cas12a and its corresponding NEBuffer r2.1 were procured from New England Biolabs (Ipswich, Massachusetts, USA), and the RPA Kit used in this study was procured from TwistDx Ltd. (Cambridge, UK). The SYBR Select Master Mix was acquired from Applied Biosystems (Waltham, Massachusetts, USA).
DDR1 promotes breast tumor growth by suppressing antitumor immunity
Oncology Reports | 27 September 2019 | DOI: https://doi.org/10.3892/or.2019.7338
Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.
Only for research and not intended for treatment of humans or animals
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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory