Description

BE4 single-base editor is the most advanced gene-editing tool, which can realize the precise replacement of a single base. There is no double-stranded break in the editing process, and the only single-stranded incision is required to realize the precise editing of a single base, which can effectively avoid genome damage in the editing process.

By fusing APOBEC1 (a cytosine deaminase) and Uracil Glycosylase Inhibitor (UGI) on Cas9n(D10A) protein of traditional CRISPR/Cas9 system, BE4 single-base editor can achieve safe, efficient, high specificity, and high fidelity base editing of C to T.

Comparison

Features

• The editing process does not rely on double-stranded break and donor templates, which can effectively reduce the genome damage in the editing process.
• A very low indel rate can significantly reduce the probability of gene inactivation.
• The highly narrow gene editing window guarantees at most two bases been edited.
• A very low off-target rate can achieve high fidelity gene editing results. 
• The editing efficiency of C to T is > 50%.
• The C to T product ratio is >80%