Protein Directed Evolution Service
Description
Protein Directed Evolution is a cutting-edge approach that mimics natural evolution mechanisms through modern molecular biology methods, enabling the creation of a diverse library of mutated homologous genes. By employing highly sensitive directed screening strategies, we can generate mutated proteins with significant changes or novel characteristics not found in nature. Directed evolution has emerged as an efficient method for molecularly modifying proteins, offering opportunities to develop proteins with enhanced properties and novel regulatory sequences.
At our protein directed evolution service, we provide a wide range of techniques to generate gene variations. By combining our advanced directed evolution technology with tailored screening techniques, we can generate gene diversity and engineer proteins with desired traits.
With our team's extensive expertise and research experience in protein and enzyme engineering, we are committed to delivering professional, rapid, and cost-effective protein evolution services that meet the unique needs of your research projects.
Features
Advanced Design: Our approach incorporates advanced design strategies, allowing for the creation of rational diversity while avoiding unnecessary mutations.
Large Library Capacity: Our libraries have a capacity exceeding 10^12, enabling the generation of rich diversity for exploration.
High Quality: Our process significantly reduces the screening burden, increasing the likelihood of obtaining useful variants with improved properties.
Service Types
- Site-Directed Mutant Library: Constructed using synthetic biology techniques, the site-directed mutant library is an effective approach for systematic research on protein properties, regulation, and function. Our site-directed mutation service offers precise design, mutation, and construction of one or multiple amino acid positions according to specific requirements. Each targeted amino acid can be mutated to any or multiple amino acids from the 20 conventional types, with precise control over their proportions in the sequence. This service includes scanning point mutation libraries, single-point mutations, multiple-point mutations, saturation mutagenesis, and conditional mutations.
- Random Mutant Library: Our random mutant service platform utilizes efficient reagents and systems for introducing random mutations into DNA sequences. The mutation frequency can be controlled at 1-10 mutations per kilobase, providing high efficiency and flexibility.
- Sequence Combination Library: The sequence combination library involves the combination, splicing, and construction of basic DNA expression elements (such as promoters, untranslated regions, signal peptides, and terminators) with different coding sequences, resulting in diverse combinations of sequence expression regulation. Researchers can recombine and splice different regulatory elements or genetic pathways according to their own designs, creating new transcriptional units, biochemical pathways, genetic pathways, or engineered bacteria.
- Truncated Library: The truncated library service is an ideal tool for structural biologists to identify the minimal stable structural domains suitable for crystallization and determine the boundary amino acid residues important for protein functionality. Truncated variants can also be obtained by systematic reduction from the N- and C-termini of the protein. Compared to site-directed mutations, truncated libraries enable cost-effective high-throughput screening of peptides and proteins of varying lengths.
