Description

Pig CRISPR Knockout Pooled Library is designed for whole-genome knockout and screening in pigs, targeting a total of 22,661 genes. The library comprises 123,951 knockout vectors, with each gene targeted by five gRNA knockout vectors. Additionally, there are 2,000 control vectors (including 2,000 targeting non-genic sequences). This library utilizes the lentiCRISPR v2 backbone, an all-in-one vector system where gRNA and Cas9 genes are on the same vector, allowing for standalone use.

• Species: Pig

• Library Type: Knockout Library

• Plasmid System: Single Plasmid System

• Virus Packaging System: Third Generation Virus Packaging System

• Target Genes: 22,661

• Number of gRNAs: 123,951

• Non-target Control sgRNAs: 2,000

• Tag: Puro

Featured Citations

Interested in seeing published research using our gRNAs?

DDR1 promotes breast tumor growth by suppressing antitumor immunity

_{Oncology Reports    |    27 September 2019    |    DOI: https://doi.org/10.3892/or.2019.7338}

_{Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.}

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory