Description

Pangenome refers to the total set of genes present in a species, distinct from individual genomes. An individual's genome represents the species' genome but does not fully capture all the genetic variation within the species. Therefore, analyses based on a single genome template may not comprehensively reflect the full genetic information at the species level, especially when studying subspecies or variants with significant differences. Variations in unique gene fragments often hold more importance than those in shared fragments. The pangenome analysis focuses on understanding the core and accessory genes, and studying species-wide differences from the perspective of unique gene sequences.

Methods for Constructing a Pangenome

There are three main methods for constructing a pangenome: Map to Pan, Iterative Assembly, and De Novo Assembly.

De Novo Assembly: This is the most classic method for constructing a pangenome or reference genome. It involves de novo assembly and annotation of multiple individual genomes separately. The assembled sequences of each individual are then compared with a reference genome to identify regions or genes that do not align. These unique genes in each individual are classified as accessory genes or non-core genes.

Workflow

1. Sample Shipment
2. Library Construction and Sequencing
3. Data Analysis
4. Report Generation
5. Post-Sale Support