Description

Mouse CRISPR Activation Library (SAM - 3 Plasmid System) is designed for whole-genome activation and screening in mice, targeting a total of 23,439 metabolic genes. The library comprises 69,225 knockout vectors, with each gene targeted by three gRNA knockout vectors. Additionally, there are 491 control vectors (including 491 targeting non-genic sequences). This library utilizes the lenti sgRNA(MS2)_puro optimized backbone, an all-in-one vector system where gRNA and Cas9 genes are on the same vector, allowing for standalone use.

• Species: Mouse

• Library Type: Activation Library

• Plasmid System: Three Plasmid System

• Virus Packaging System: Third Generation Virus Packaging System

• Target Genes: 23,439

• Number of gRNAs: 69,225

• Non-target Control sgRNAs: 491

• Tag: Puro; BSD; Hygro

Featured Citations

Interested in seeing published research using our gRNAs?

DDR1 promotes breast tumor growth by suppressing antitumor immunity

_{Oncology Reports    |    27 September 2019    |    DOI: https://doi.org/10.3892/or.2019.7338}

_{Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.}

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory