Description

This knockout library is suitable for whole-genome knockout and screening in humans, targeting 19,050 human genes and comprising a total of 65,383 knockout vectors. Each gene is targeted by three gRNA knockout vectors, with an additional 1,000 control vectors targeting non-genomic sequences. The library uses the LentiGuide-Puro backbone and operates as a dual-vector system, expressing only the gRNA. The Cas9 gene is on a separate vector and needs to be used in conjunction.

Species: Human 

Library Type: Knockout Library 

Plasmid System: Dual Vector System 

Viral Packaging System: Third Generation Viral Packaging System 

Target Genes: 19,050 

Number of gRNAs: 65,383 

Number of Non-Target Control sgRNAs: 1,000 

Tag: Puro

Featured Citations

Interested in seeing published research using our gRNAs?

DDR1 promotes breast tumor growth by suppressing antitumor immunity

_{Oncology Reports    |    27 September 2019    |    DOI: https://doi.org/10.3892/or.2019.7338}

_{Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.}

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory