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Glutamate Dehydrogenase | 9001-46-1
More Details
DESCRIPTION | |
Catalog No.: | 9001-46-1 |
Appearance: | White amorphous powder |
Source: | Microorganism |
Enzyme Commission Number: | EC 1.4.1.2 |
CAS Number: | 9001-46-1 |
Storage temperature: | -20℃ |
Specific activity: | ≥ 400U/mg protein |
Unit definition: | One unit will convert one micromole of α- ketoglutarate to L-glutamate per min at pH 8.3 at 30℃. |
PROPERTIES | |
Molecular weight: | 260 kDa (Gel filtration) |
Isoelectric point: | 5.6 |
Michaelis constant: | 9.5×10-3 M (NH ) 3 |
5.0×10-3M (α-Ketoglutarate) | |
8.4×10-5M (NADH) | |
Optimum pH: | 8.5(α-KG→L-Glu) |
Optimum temperature: | 45℃ |
pH Stability: | 5.0~11.0 (25℃, 20hr) |
Thermal stability: | < 60℃ (pH 8.3, 10min) |
Inhibitors: | Co2+ , Fe3+,NEM,Proclin,SDS |
Only for research and not intended for treatment of humans or animals