For the synthesis of gene fragments up to 200kb, please visit Ultra-long Gene Synthesis for more details.

Description

After over twenty years of dedicated research in the field of gene synthesis, we have achieved this groundbreaking milestone. We have successfully dismantled the traditional gene assembly process of PCA-PCR-Gibson, replacing it with a streamlined single-step innovation: a one-step gene assembly technology spanning from oligo to plasmid. Our approach integrates second-generation sequencing QC and state-of-the-art fully automated equipment, ushering in a comprehensive revolution in delivery cycles and synthesis costs. Moreover, we've pioneered rapid synthesis technology for 10Kb gene fragments, substantially slashing time requirements compared to conventional methods while dramatically reducing synthesis costs. Remarkably, our cutting-edge techniques enable gene synthesis of up to 200Kb in length, marking a significant leap forward in the field of genetic engineering.

Features

• Single-step gene assembly technology: Our single-step gene assembly technology simplifies the traditional laborious PCA-PCR-Gibson three-step synthesis process into one. This innovation significantly enhances production efficiency and synthesis speed, saving customers valuable time and costs.
• Cost reduction: Through process optimization and technological innovation, we have successfully reduced the cost of gene synthesis. This feature provides customers with an affordable option, making gene synthesis more attractive.
• High success rate guarantee: Our technology ensures a high success rate during the synthesis process, effectively reducing the risk of failure and additional costs. Customers can confidently rely on our service to obtain reliable synthesis results.
• Rapid synthesis technology: We have developed rapid synthesis technology that significantly shortens synthesis time and improves production efficiency. This allows customers to obtain the gene fragments they need more quickly, accelerating their research and project progress.
• Long fragment synthesis capability: Our technology has the ability to synthesize gene fragments up to 200Kb in length, meeting customers' demands for synthesizing large gene fragments. This provides possibilities for complex genetic engineering projects and expands the scope of customers' research and applications.

Pricing

Assuming all sequences are codon-optimized for protein expression and high GC content and repeat sequences can be modified for easy manipulation and synthesis to meet required turnaround times.

In the event that the construct is toxic to E.coli or will not clone, the customer will be immediately contacted and an alternative strategy will be agreed upon to generate the desired construct (an additional fee may be added).

Delivery Specs

• 2-5ug of plasmid DNA containing the synthesized gene.
• A stab of E.coli harboring the plasmid and gene.
• Certificate of Analysis for each construct.
• Sequence trace files, double-strand sequence verification.
• Text and Doc files of the gene sequence alone and combined with plasmid.

Subcloning

We can custom clone each gene into a commercially available E.coli vector at $50/each, and clone it into a non-commercial vector at $100/each. Sequences larger than 5kb are quoted on an individual basis. Complexity may also result in a price change.

Related: Ultra-long Gene Synthesis, Plasmid DNA Preparation Service

SBS Genetech is recognized as one of the global major leading industry players in Gene Synthesis by third-party market researchers. For more details, please visit GENE SYNTHESIS MARKET SIZE & SHARE ANALYSIS - GROWTH TRENDS & FORECASTS (2023 - 2028).

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory