Description

Funcbeads™ Hydroxyl Oligo (dT) Magnetic Beads can be rapidly and completely separated when a magnetic field is present. Utilizing standard hybridization conditions, polyadenylated RNA (poly-A + RNA) can be easily bound onto the oligo (dT) set, and other RNAs (rRNA and tRNA) do not contain poly A+ sequences and therefore will not bind to oligo (dT) magnetic beads. Therefore, the isolated mRNA has high purity and can be used directly in downstream applications such as cloning and expression analysis.

Uniformity of particle size and composition

Tightly controlled production processes result in core-shell beads which are uniform and spherical. The silica shell provides a high content of silanol surface groups and encloses the magnetic core, as confirmed by Fourier-Transformed Infrared (FT-IR) spectroscopy. The submicron size allows for a high surface area and abundant binding sites. The narrow particle size distribution provides uniformity and consistency of the beads. The high zeta-potential values indicate a high surface charge, allowing for monodispersity and stable suspensions (long gravity settling time/low sedimentation rate) which contribute to ease of handling during use. As a result, VirusMag™ Magnetic Beads delivers a high-performance surface and regular morphology to optimize binding efficiency and reduce variability.

Principle

An appropriate lysis buffer is first added to the sample, followed by incubation with beads. The beads with bound DNA/RNA are easily pulled to the side of the test tube by applying a magnetic field, and unbound material is removed by aspiration. The magnetic separation also facilitates simple washing and elution of the isolated nucleic acids. The protocol and buffer systems used should be optimized relative to your specific sample type and target material.

Storage

Minimum shelf life in one year at 2-8°C.

Related: Funcbeads™ Hydroxyl Magnetic Beads​, Funcbeads™ Amino Magnetic Beads, Funcbeads™ SA Magnetic Beads, Funcbeads™ Carboxyl Magnetic Beads, Funcbeads™ Anti-FLAG Magnetic Beads

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory