Description

Funcbeads™ Hydroxyl Magnetic Beads are composed of silica with coated superparamagnetic iron oxide particles. The beads are designed for efficient nucleic acid (DNA/RNA) isolation and purification for a range of samples, from biofluids to liquid biopsy. The beads provide an optimal content of ferric oxide for high magnetization in the presence of a magnetic field and low sedimentation rate. At the same time, they provide good binding capacity. The surface functional group is -OH.

The beads are compatible with chaotropic salt chemistry. They are supplied at a concentration of 50 mg/mL with monodispersed suspension in water. The beads should be resuspended (e.g. vortex) to a homogenous suspension prior to use.

Specification

Uniformity of particle size and composition

Tightly controlled production processes result in core-shell beads which are uniform and spherical. The silica shell provides a high content of silanol surface groups and encloses the magnetic core, as confirmed by Fourier-Transformed Infrared (FT-IR) spectroscopy. The submicron size allows for a high surface area and abundant binding sites. The narrow particle size distribution provides uniformity and consistency of the beads. The high zeta-potential values indicate a high surface charge, allowing for monodispersity and stable suspensions (long gravity settling time/low sedimentation rate) which contribute to ease of handling during use. As a result, VirusMag™ Magnetic Beads delivers a high-performance surface and regular morphology to optimize binding efficiency and reduce variability.

Principle

An appropriate lysis buffer is first added to the sample, followed by incubation with beads. The beads with bound DNA/RNA are easily pulled to the side of the test tube by applying a magnetic field, and unbound material is removed by aspiration. The magnetic separation also facilitates simple washing and elution of the isolated nucleic acids. The protocol and buffer systems used should be optimized relative to your specific sample type and target material.

Storage

Minimum shelf life in one year at 2-8°C.

Related: Funcbeads™ Hydroxyl Oligo (dT) Magnetic Beads, Funcbeads™ Amino Magnetic Beads, Funcbeads™ SA Magnetic Beads, Funcbeads™ Carboxyl Magnetic Beads, Funcbeads™ Anti-FLAG Magnetic Beads

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory