
Double-Quenched Probes
Description
With the increase in probe length, the quenching efficiency of single-quenched probes diminishes, while the emergence of double-quenched probes effectively addresses this issue. Conventional probes carry a fluorescent moiety at the 5' end and a quencher at the 3' end. SBS Genetech's introduction of double-quenched probes involves the addition of an extra quencher moiety within the conventional probe, reducing fluorescence "leakage". This enhancement enables the attainment of lower fluorescent background signals in experiments, thereby enhancing detection sensitivity.
Advantages
The utilization of high-quality fluorescent probes can significantly enhance assay efficiency and detection accuracy. Evaluation criteria for these probes primarily encompass selectivity, sensitivity, response speed, fluorescence enhancement factor, stability, and biocompatibility. Notably, conventional nucleic acid probes exhibit only one type of fluorescence quenching effect! The effectiveness of the quencher moiety in extinguishing fluorescence relies on distance; proximity results in efficient quenching, whereas greater separation leads to diminished quenching and heightened background fluorescence signals. Double-quenched probes offer a compelling solution to this "distance" challenge, thereby markedly improving qPCR assays!
- The dual-quenching mechanism effectively reduces background signals, enhancing sensitivity.
- It reduces fluorescence signal crosstalk in multi-channel detection, ensuring compatibility.
- Probe lengths can be designed up to 40 nucleotides, offering improved selectivity.
Comparison
Performance | Conventional Probe | Double-Quenched Probe |
---|---|---|
Applicable Length | <30nt | <45nt |
Reduced Background Signal | √ | √√ |
Sensitivity | √ | √√ |
Compatibility with Multiplex PCR | √ | √√ |
Note:
- "√" denotes the presence of the feature.
- "√√" indicates a higher level or enhanced feature compared to the standard probe.

Selected Published Papers
SBS Genetech supplies high-quality oligonucleotides, empowering researchers to publish over 800 papers in prestigious journals such as Nucleic Acids Research, Biosensors and Bioelectronics, and Blood
Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level
Nucleic Acids Research | 01 Jan 2004 | DOI: https://doi.org/10.1093/nar/gnh100
All the DNA sequences were custom synthesized from SBS Genetech Co. Ltd. (Beijing, China). These include the DRE element sequence (ACCGAC), 5′-NH 2 -GATATACT ACCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTGTACTCAAG-5′; the DRE element sequence (GCCGAC), 5′-NH 2 -GATATACT GCCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGACGGCTGTACTCAAG-5′; the ERE element (GCCGCC), 5′-NH 2 -CGCAGACATA GCCGCC ATTT-3′, and its complementary ssDNA, 3′-GCGTCTGTATCGGCGGTAAA-5′; the mutant DRE element sequence (ACCGAG), 5′-NH 2 -GATATACT ACCGAG ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTCTACTCAAG-5′ (the element sequences are underlined).
Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant
Blood | 01 March 2004 | DOI: https://doi.org/10.1182/blood-2003-08-2828
To assess Hsp70L1 expression in DCs, 6-day DCs generated from human peripheral monocytes were stimulated at 5 × 105 cells/mL for 8 hours with 1 μg/mL lipopolysaccharide (LPS) (Sigma), 30 μg/mL CpG oligonucleotides (5′-TCGTCGTTCCCCCCCCCC-CC-3′; SBS Genetech, Beijing, China)
A thermodynamic overview of naturally occurring intramolecular DNA quadruplexes
Nucleic Acids Research | 30 Aug 2008 | DOI: https://doi.org/10.1093/nar/gkn543
HPLC purified oligonucleotides were procured from SBS Genetech, China. The concentrations of these oligonucleotides were calculated by extrapolation of tabulated values of the monomer bases and dimers at 25°C using procedures reported earlier
Quartz crystal microbalance detection of protein amplified by nicked circling, rolling circle amplification and biocatalytic precipitation
Biosensors and Bioelectronics | 31 Oct 2014 | DOI: https://doi.org/10.1016/j.bios.2014.10.055
Deoxyribonucleoside 5′-triphosphates (dNTPs) mixture and all oligonucleotides as depicted in Table S1 of Supporting information were purchased from SBS Genetech. Co. Ltd. (China).
Electrochemical aptasensor based on one-step synthesis of Cu2O@aptamer nanospheres for sensitive thrombin detection
Sensors and Actuators B: Chemical | 10 June 2015 | DOI: https://doi.org/10.1016/j.snb.2015.05.089
Blood sera and aptamer were acquired from SBS Genetech Co. Ltd. (Beijing, China) and has the following sequences: 5′-TCTCTCAGTCCGTGGTAGGGCAGGGTTGGGGTGACT-3′......
Carbon-based nanocomposites with aptamer-templated silver nanoclusters for the highly sensitive and selective detection of platelet-derived growth factor
Biosensors and Bioelectronics | 16 Nov 2016 | DOI: https://doi.org/10.1016/j.bios.2016.11.019
Aptamer was obtained from SBS Genetech Co., Ltd. (Beijing, China), and the sequence was: 5′-CAG GCT ACG GCA CGT AGA GCA TCA CCA TGA TCC
Fe(III)-based metal–organic framework-derived core–shell nanostructure: Sensitive electrochemical platform for high trace determination of heavy metal ions
Biosensors and Bioelectronics | 17 Mar 2017 | DOI: https://doi.org/10.1016/j.bios.2017.03.014
Aptamer strands were obtained from SBS Genetech Co. Ltd (Beijing, China).
A CRISPR/Cas12a-based fluorescence aptasensor for the rapid and sensitive detection of ampicillin
International Journal of Biological Macromolecules | 9 June 2023 | DOI: https://doi.org/10.1016/j.ijbiomac.2023.125211
The oligonucleotides employed in this experiment were procured from Beijing SBS Genetech Co. Ltd. (China). The sequences of the reporter and three aptamers were obtained in accordance with their relevant references [36,37], while crRNA and activator DNA sequences were designed in our laboratory.
Smart co-delivery of plasmid DNA and doxorubicin using MCM-chitosan-PEG polymerization functionalized with MUC-1 aptamer against breast cancer
Biomedicine & Pharmacotherapy | 19 March 2024 | DOI: https://doi.org/10.1016/j.biopha.2024.116465
The aptamer used (sequence: 5′-GCCCGCCGTGGCTGGGTCTTCCTTGGTCGGTCTACAAAAAAAAAA-SH-3′) was obtained from SBS Genetech Co. Ltd.
Exploring aptamer-aTF sandwich and CRISPR-Cas12a methods for sensitive L-lactate biosensing in human serum and saliva
Sensors and Actuators B: Chemical | 28 November 2024 | DOI: https://doi.org/10.1016/j.snb.2024.137015
The oligonucleotides utilised in this study were obtained from Beijing SBS Genetech Co. Ltd. (Beijing, China). The sequences of the dsDNA, reporter, and aptamers were obtained following their relevant references while gRNA...
Nucleic acid-free aptamer-CRISPR/Cas14 biosensor for prosthetic joint infection rapid detection
Sensors and Actuators Reports | 24 March 2025 | DOI: https://doi.org/10.1016/j.snr.2025.100318
Nucleotide sequences were synthesized by Beijing SBS Genetech Co., Ltd.
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