Description

Compared to traditional in vivo protein expression methods, cell-free protein expression enables rapid and low-cost protein production. Due to its open reaction environment, free from cellular structures and physiological constraints, it can easily complete all intracellular metabolic and synthetic processes in ordinary reaction vessels. This results in high versatility and flexibility, facilitating efficient substrate handling, precise online reaction monitoring, and direct process optimization.

Leveraging the extensive experience of our professional technical team in DNA synthesis and molecular cloning, combined with our multifunctional and efficient cell-free protein expression platform, we provide unique and comprehensive solutions for customized projects or specialized applications for our clients.

Difficult-to-Express Proteins and Protein Drugs

The cell-free protein expression platform has been successfully used to synthesize proteins of various molecular weights, ranging from single-domain to multi-domain, and from single subunit to multi-subunit protein complexes, with molecular weights from 10 kDa to 160 kDa. The cell-free protein expression system is capable of handling these and has been validated for activity. Some examples of successfully expressed proteins include disulfide-bonded proteins, such as human urinary trypsin inhibitor UTI (6 pairs) and tissue plasminogen activator tPA (17 pairs); membrane proteins, such as aquaporin aqpZ and G protein-coupled receptor CXCR4; antibody proteins, such as scFv, Fab, and IgG; and cytotoxic proteins, such as apoptotic caspase-1 and ribonuclease Onconase, among others.