In the vast realms of molecular biology and genomics, the ability to precisely label and visualize nucleic acids (DNA and RNA) is a critical skill. Whether unraveling the intrinsic mysteries of gene expression or developing cutting-edge diagnostic methods, these tiny molecules hold the key to unlocking the secrets of life. Among the various labeling reagents, CY3 Phosphoramidite (Cyanine3 phosphoramidite) shines like a brilliant orange-red beacon, guiding researchers to incorporate this vibrant fluorescent dye into oligonucleotide (short DNA or RNA) sequences with pinpoint accuracy.
A Captivating Orange-Red Signal Flare
CY3 Phosphoramidite is a specialized reagent that allows for the site-specific attachment of the fluorescent cyanine dye Cy3 to oligonucleotides. This bright dye boasts an absorption maximum around 550 nm and an emission maximum around 570 nm, emitting a captivating orange-red fluorescence that can be easily detected and quantified.
CY3-labeled oligonucleotides are compatible with dark quenchers, such as BHQ-1, enabling the creation of highly sensitive fluorescence-based probes and assays. In these systems, the dye's fluorescence is efficiently quenched until a specific event (e.g., hybridization) triggers the separation of the dye and quencher, releasing a detectable fluorescent signal.
A Versatile Beacon for Diverse Applications
The versatility of CY3 Phosphoramidite is evident in its wide range of applications. Whether in DNA sequencing, fluorescence in situ hybridization (FISH), microarray analysis, or real-time PCR, CY3-labeled oligonucleotides serve as powerful fluorescent probes and primers, enabling researchers to visualize and quantify target sequences with unparalleled accuracy.
Moreover, incorporating the Cy3 dye into oligonucleotides is invaluable for developing fluorescence-based assays and sensors. These innovative tools leverage the unique properties of the Cy3 dye to detect and monitor various molecular interactions, providing insights into biological processes and paving the way for novel diagnostic and therapeutic approaches.
Precise and Reliable Synthesis Protocols
The power of CY3 Phosphoramidite is undeniable, but its successful incorporation into oligonucleotides requires careful handling and synthesis considerations. Proper storage and handling techniques are crucial to prevent oxidation and maintain the reagent's performance, ensuring consistent and reliable results.
During the oligonucleotide synthesis process, specific coupling conditions, such as extended coupling times, may be required to ensure efficient incorporation of the Cy3 dye. Additionally, deprotection protocols must be strictly followed, avoiding elevated temperatures (>65°C) that could lead to dye degradation, particularly for the related Cy5 dye.
Illuminating the Path to Molecular Understanding
As molecular biology continues to advance, the demand for sophisticated labeling and visualization techniques will only grow. CY3 Phosphoramidite represents a significant step forward in this direction, enabling researchers to create highly specific and sensitive fluorescent probes and assays.
Whether unraveling the complexities of gene expression or developing cutting-edge diagnostic tools, the brilliant orange-red guiding light of CY3 Phosphoramidite is poised to illuminate the path towards a deeper understanding of the molecular world. With its versatility, compatibility with state-of-the-art quenching technologies, and proven track record in diverse applications, this remarkable reagent is empowering scientists to explore new frontiers and unlock the intricate secrets hidden within the dance of nucleic acids.
High-Quality Fluorescently Labeled Oligonucleotide Synthesis: Choose SBS Genetech
When selecting reagents for fluorescently labeled oligonucleotide synthesis, quality and reliability are paramount. As an industry-leading biotechnology company, SBS Genetech is committed to providing researchers with high-quality fluorescently labeled DNA Synthesis Products. We utilize advanced synthesis technology and rigorous quality control systems to ensure that each batch of products boasts exceptional purity and stability.
Our Advantages Include:
- Strict Quality Control: Each batch undergoes rigorous quality testing, including spectroscopic analysis and mass spectrometry verification, to ensure its fluorescent performance and chemical stability.
- Custom Services: We offer custom synthesis services tailored to customer needs, meeting various experimental requirements, including special modifications, specific sequences, and labels.
- Technical Support: Our expert team provides comprehensive technical support and consultation services to help customers resolve various issues encountered during experiments.
Extensive Application Areas
Our labeled oligonucleotides are widely used in the following research areas:
- Gene Expression Analysis: Highly sensitive qPCR for precise measurement of gene expression levels.
- Pathogen Detection: Rapid and accurate detection of various pathogens, including viruses, bacteria, and fungi.
- Genomic Research: Used in genome sequencing, SNP detection, and mutation analysis, driving advancements in genomics research.
- Molecular Interaction Studies: Utilizing FRET technology to study molecular interactions, revealing dynamic mechanisms of biomolecules.
Welcome to Order
At SBS Genetech, we are dedicated to advancing the frontiers of scientific research. We understand the complexity and rigor of scientific work, and it is our responsibility and mission to provide high-quality reagents and excellent service. If you need high-quality fluorescent labeling reagents, please feel free to contact us. We are committed to providing you with superior products and professional support, helping you achieve greater success in your research endeavors.
Choosing SBS Genetech means choosing excellence and reliability. Let us work together to contribute to the bright future of scientific research.