The science of genetics has always been rooted in understanding the intricate dance of DNA and RNA, which ultimately directs life's phenomenally diverse expressions. SBS Genetech, with its series of reverse transcriptases, takes a leap forward, introducing optimized enzymes that promise to enhance the quality and efficacy of reverse transcription and amplification processes. In this blog, let’s delve into the advanced offerings of four SBS Genetech’s reverse transcriptases.
Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
Creating a breakthrough in the world of genetics, Thermo-stable M-MLV Reverse Transcriptase (RNase H-) unlocks the door to more efficient and stable cDNA synthesis. With RNase H activity minimized through multiple point mutations, this enzyme fosters reduced RNA degradation during reverse transcription, facilitating an enriched yield of first-strand cDNA. Notably, its enhanced thermal stability, with an optimal reaction temperature of 50°C, allows researchers to attain full-length cDNA with relative ease.
Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
Building upon the foundation of its predecessor, the Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-) pushes the boundaries with an enhanced thermal stability that extends the enzyme's activity up to a striking 65°C. This thermal resilience enables significant unfolding of RNA’s secondary structure at high temperatures, promoting the amplification of complex RNA templates and thereby, amplifying both the length and yield of cDNA. The boosted sensitivity of subsequent detection is an added feather in its cap, heralding improved accuracy in applications like RT-PCR.
Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-) stands out with its exceptional reverse transcription efficiency and thermal resistance, extending its transcription temperature up to 72°C. Originating from point mutations based on the wild-type M-MLV reverse transcriptase gene, and expressed and purified via specially engineered bacteria, it significantly enhances the tolerance to the complex secondary structures of RNA. With a protein structure intact and akin to the wild type, it sustains polymerase activity while boasting high synthesis capacity and commendable thermal stability.
Warm Activated Reverse Transcriptase
Warm Activated Reverse Transcriptase, an RNase H-deficient variant of M-MLV, finds its uniqueness in utilizing nucleic acid adapter technology, which covalently binds to M-MLV and inhibits its activity below 31°C, ensuring enhanced product specificity. With gene modification and recombinant techniques under its belt, the enzyme's optimal reaction temperature is elevated to 50°C, thereby improving its extension capability and making it apt for elongated cDNA synthesis.
Conclusion
Embarking on a journey with these sophisticated reverse transcriptases from SBS Genetech, researchers and scientists can anticipate a future where genetic transcription and amplification processes are not only more efficient but also yield higher fidelity results. The nuanced modifications and enhancements introduced in each of these enzymes ensure that the reverse transcription is more reliable, stable, and adaptable to various research needs and conditions.
Since 2000, SBS Genetech has been at the forefront of providing solutions in life sciences. We offer safer, superior quality, and more cost-effective products to preeminent researchers in nearly 60 countries, empowering them to make new discoveries in biology. Our products have been widely utilized in academic research, with results often published in leading academic journals like Science, Cell, Cancer Cell, and Cell Metabolism. We firmly believe that through continuous innovation and research, we will continually infuse new vitality and possibilities into the field of life sciences.