Enzymes for Recombinase Polymerase Amplification (RPA) are indispensable components in the revolutionary molecular biology technique known as Recombinase Polymerase Amplification (RPA). This method has garnered immense popularity due to its ability to rapidly amplify DNA or RNA targets under isothermal conditions, typically between 37-42°C. Offering simplicity, speed, and versatility, RPA finds applications across various fields including diagnostics, research, and biotechnology.

Let's delve deeper into the key reagents commonly utilized in RPA:

• T4 UvsX Recombinase: Derived from the T4 bacteriophage, T4 UvsX Recombinase is a vital enzyme in RPA. It plays a pivotal role in initiating the amplification process by facilitating the strand exchange step. UvsX enables the binding of UvsX to single-stranded DNA (ssDNA), a crucial step in amplification initiation.
• T4 UvsY Protein: Another essential protein derived from the T4 bacteriophage, T4 UvsY Protein aids in assembling the presynaptic filament. By promoting the binding of UvsX to ssDNA and stimulating its recombinase activity, UvsY facilitates efficient amplification in RPA.
• T4 Gene 32 Protein: Encoded by the T4 bacteriophage, T4 Gene 32 Protein serves as a single-stranded DNA-binding protein (SSB). In RPA, it plays a critical role in stabilizing ssDNA intermediates, preventing their degradation, and facilitating subsequent steps in the amplification process.
• Bsu DNA Polymerase: Also known as Bacillus subtilis DNA Polymerase, Bsu DNA Polymerase is a DNA-dependent DNA polymerase enzyme derived from Bacillus subtilis. It is employed in RPA to synthesize complementary DNA strands during the amplification process.
• Sau DNA Polymerase: Derived from Staphylococcus aureus, Sau DNA Polymerase is another DNA-dependent DNA polymerase enzyme utilized in RPA for amplifying DNA targets. Functioning similarly to Bsu DNA Polymerase, it synthesizes new DNA strands during the amplification process.
• Exonuclease III: Exonuclease III is an enzyme catalyzing the removal of nucleotides from DNA ends. In RPA, it aids in enhancing the specificity of the amplification reaction by eliminating any unwanted DNA fragments or primers.
• Bst DNA/RNA Polymerase: Bst (Bacillus stearothermophilus) DNA/RNA Polymerase is an enzyme capable of synthesizing DNA from RNA templates (reverse transcription) as well as DNA from DNA templates. Widely used in RPA, it amplifies RNA targets directly or generates complementary DNA (cDNA) from RNA templates for subsequent amplification.
• Endonuclease IV: Endonuclease IV is an enzyme that cleaves DNA at apurinic/apyrimidinic (AP) sites. It finds application in RPA by creating nicks or breaks in DNA strands, facilitating the initiation of DNA amplification.

At SBS Genetech, leveraging over two decades of expertise in genetic engineering, protein engineering, and fermentation technologies, we excel in the efficient and cost-effective production of these crucial enzymes. Our seasoned team meticulously controls the production processes, ensuring the enzymes' high purity and activity.

Moreover, we offer these enzymes in lyophilized form, harnessing their long-term stability and convenient transportability at room temperature without compromising their activity. This service provides our clients with enhanced convenience and flexibility, both domestically and internationally.

With our production and supply chain advantages, we guarantee our clients access to high-performance, high-quality enzyme products. We also provide tailored solutions to meet specific research and production requirements. Committed to delivering top-notch products and services, we empower our clients to achieve greater success in the realm of scientific research.