Loop-mediated isothermal amplification of DNA is a powerful molecular biology technique used to amplify specific DNA sequences under isothermal conditions. Unlike traditional polymerase chain reaction (PCR), which requires thermal cycling through different temperature steps, LAMP amplifies DNA at a constant temperature, typically between 60-65°C.
The LAMP reaction relies on a strand-displacing DNA polymerase, usually Bst DNA polymerase, and a set of four to six primers that recognize multiple regions within the target DNA sequence. These primers initiate DNA synthesis in a cyclic manner, leading to the formation of stem-loop DNA structures known as amplicons. These structures serve as templates for further amplification, resulting in exponential accumulation of DNA.
Key features of LAMP include:
- Isothermal amplification: The reaction is conducted at a constant temperature, eliminating the need for a thermal cycler and simplifying the amplification process.
- High specificity: LAMP employs multiple primers that recognize different regions of the target sequence, enhancing specificity and reducing the likelihood of nonspecific amplification.
- Efficiency: LAMP is highly efficient and can amplify target DNA rapidly, typically within 30-60 minutes.
- Visual detection: The amplification products can often be detected visually by the naked eye through turbidity caused by magnesium pyrophosphate precipitation or by the addition of DNA intercalating dyes that produce a color change.
- Robustness: LAMP is less sensitive to inhibitors present in crude samples compared to PCR, making it suitable for direct amplification from complex biological samples such as blood, urine, or saliva.
LAMP has found widespread applications in various fields including clinical diagnostics, food safety testing, environmental monitoring, and point-of-care diagnostics due to its simplicity, rapidity, and versatility.
At SBS Genetech, our pioneering spirit drives us to continually enhance our offerings in loop-mediated isothermal amplification (LAMP) technologies. Our Bst P DNA/RNA Polymerase stand as testament to our dedication to innovation and excellence.
Bst P DNA/RNA Polymerase represents a significant advancement, meticulously crafted through enzyme electronic re-structuring and evolution screening. This upgraded version, designed for LAMP or RT-LAMP amplification of DNA or RNA, offers unparalleled performance and versatility.
Highlighted in the esteemed Science Journal on April 13, 2023, our glycerol-free Bst P DNA/RNA Polymerase underscores our commitment to pushing the boundaries of molecular biology research. This recognition reaffirms our position at the forefront of scientific advancement and underscores the transformative potential of our technologies.
Key features of the Bst P DNA/RNA Polymerase include:
- Integration of a hot start Aptamer, ensuring precise control over enzyme activity, even at low temperatures, and facilitating rapid activation for efficient amplification.
- Elevated reaction temperature to 70°C, minimizing primer dimer formation, enhancing amplification specificity, and ensuring efficient nucleic acid release from crude samples.
- Incorporation of Helicase, enabling Premium LAMP amplification (pLAMP) without the need for F3/B3 primers, while also assisting in strand unwinding to improve amplification uniformity.
- Complementing our enzyme portfolio is our customized lyophilized microbead service, offering tailored solutions to meet the unique requirements of your research projects. With customized primers and reaction volumes, we empower researchers to optimize experiments with precision and efficiency.
For more information on our Bst P DNA/RNA Polymerase and other groundbreaking products, visit the Science Journal article dated April 13, 2023, or contact us to discover how SBS Genetech can accelerate your research journey.
Related:
- Bst DNA Polymerase Large Fragment
- Bst DNA Polymerase (comparable to 2.0)
- Bst Polymerase (comparable to 3.0)
- Bst Polymerase (glycerol-free)
- Bst DNA/RNA Polymerase (with superior reverse transcription activity)
- Bst DNA/RNA Polymerase (glycerol-free)
- Bst P DNA/RNA Polymerase (glycerol-free) (with superior reverse transcription activity & hot-start property)