In the ever-evolving landscape of molecular biology and genomics, the ability to precisely label and visualize nucleic acids (DNA and RNA) has become an indispensable tool. From unraveling the intricate mechanisms of gene expression to developing cutting-edge diagnostic assays, these tiny molecules hold the key to unlocking the secrets of life itself. Among the arsenal of labeling reagents, CY5 Phosphoramidite stands out as a beacon of brilliance, enabling researchers to incorporate a vibrant red fluorescent dye into their oligonucleotide sequences.

A Radiant Red Fluorescent Beacon

CY5 Phosphoramidite is a specialized reagent that allows for the site-specific attachment of the cyanine dye Cy5 to oligonucleotides. This bright fluorescent dye boasts an absorption maximum around 650 nanometers and an emission maximum around 670 nanometers, emitting a radiant red fluorescence that can be easily detected and quantified.

One of the key advantages of Cy5-labeled oligonucleotides is their compatibility with dark quenchers, such as BHQ-2 and Iowa Black RQ. This unique property enables the creation of highly sensitive fluorescence resonance energy transfer (FRET) assays and probes, where the dye's fluorescence is efficiently quenched until a specific event triggers its release, resulting in a detectable signal.

Versatile Applications in Molecular Exploration

The versatility of CY5 Phosphoramidite is evident in its wide range of applications across various fields of molecular biology and genomics. In DNA sequencing, FISH (fluorescence in situ hybridization), microarray analysis, and real-time PCR, Cy5-labeled oligonucleotides serve as powerful fluorescent probes and primers, enabling researchers to visualize and quantify target sequences with unparalleled accuracy.

Moreover, the incorporation of the Cy5 dye into oligonucleotides has proven invaluable in the development of FRET-based assays and sensors. These innovative tools leverage the unique properties of the Cy5 dye to detect and monitor various molecular interactions, providing insights into biological processes and enabling the development of novel diagnostic and therapeutic approaches.

Precision and Reliability in Synthesis

While the power of CY5 Phosphoramidite is undeniable, its successful incorporation into oligonucleotides requires careful handling and synthesis considerations. Proper storage and handling techniques are crucial to prevent oxidation and maintain the reagent's performance and stability, ensuring consistent and reliable results.

During the oligonucleotide synthesis process, specific coupling conditions, such as extended coupling times, may be required to ensure efficient incorporation of the Cy5 dye. Additionally, deprotection protocols must be carefully followed, avoiding elevated temperatures that could lead to dye degradation, as Cy5 is particularly susceptible to this issue.

Illuminating the Path to Molecular Understanding

As the field of molecular biology continues to advance, the demand for sophisticated labeling and visualization techniques will only grow. CY5 Phosphoramidite represents a significant step forward in this direction, enabling researchers to create highly specific and sensitive fluorescent probes and assays.

From unraveling the complexities of gene expression to developing cutting-edge diagnostic tools, the radiant red fluorescent beacon of CY5 Phosphoramidite is poised to illuminate the path towards a deeper understanding of the molecular world. With its versatility, compatibility with state-of-the-art quenching technologies, and proven track record in various applications, this remarkable reagent is empowering scientists to explore new frontiers and unlock the secrets hidden within the intricate dance of nucleic acids.

High-Quality Fluorescently Labeled Oligonucleotide Synthesis: Choose SBS Genetech

When selecting reagents for fluorescently labeled oligonucleotide synthesis, quality and reliability are paramount. As an industry-leading biotechnology company, SBS Genetech is committed to providing researchers with high-quality fluorescently labeled DNA Synthesis Products. We utilize advanced synthesis technology and rigorous quality control systems to ensure that each batch of products boasts exceptional purity and stability.

Our Advantages Include:

• Strict Quality Control: Each batch undergoes rigorous quality testing, including spectroscopic analysis and mass spectrometry verification, to ensure its fluorescent performance and chemical stability.
• Custom Services: We offer custom synthesis services tailored to customer needs, meeting various experimental requirements, including special modifications, specific sequences, and labels.
• Technical Support: Our expert team provides comprehensive technical support and consultation services to help customers resolve various issues encountered during experiments.

Extensive Application Areas

Our labeled oligonucleotides are widely used in the following research areas:

• Gene Expression Analysis: Highly sensitive qPCR for precise measurement of gene expression levels.
• Pathogen Detection: Rapid and accurate detection of various pathogens, including viruses, bacteria, and fungi.
• Genomic Research: Used in genome sequencing, SNP detection, and mutation analysis, driving advancements in genomics research.
• Molecular Interaction Studies: Utilizing FRET technology to study molecular interactions, revealing dynamic mechanisms of biomolecules.

Welcome to Order

At SBS Genetech, we are dedicated to advancing the frontiers of scientific research. We understand the complexity and rigor of scientific work, and it is our responsibility and mission to provide high-quality reagents and excellent service. If you need high-quality fluorescent labeling reagents, please feel free to contact us. We are committed to providing you with superior products and professional support, helping you achieve greater success in your research endeavors.

Choosing SBS Genetech means choosing excellence and reliability. Let us work together to contribute to the bright future of scientific research.