In the realm of molecular biology, the conversion of RNA into complementary DNA (cDNA) is a fundamental process, often facilitated by reverse transcriptase enzymes. Among the commonly used enzymes for this purpose are AMV RT (Avian Myeloblastosis Virus Reverse Transcriptase) and MMLV RT (Moloney Murine Leukemia Virus Reverse Transcriptase). While both enzymes share the overarching goal of cDNA synthesis, they exhibit notable differences that warrant exploration.

• Source and Origin: AMV RT hails from the Avian Myeloblastosis Virus, while MMLV RT finds its origin in the Moloney Murine Leukemia Virus. These viral sources provide the enzymes with their catalytic properties and define their characteristics.
• Thermostability: One significant discrepancy lies in thermostability. MMLV RT boasts higher resilience to temperature fluctuations compared to its counterpart, AMV RT. This attribute is crucial in experimental setups where maintaining enzyme activity at elevated temperatures is imperative.
• Processivity: Processivity, the enzyme's ability to remain attached to the template nucleic acid strand during synthesis, differs between the two. MMLV RT tends to exhibit higher processivity, enabling efficient cDNA synthesis, particularly for longer RNA templates.
• Temperature Optima: Each enzyme harbors distinct optimal temperature ranges for activity. AMV RT generally operates optimally at higher temperatures than MMLV RT. This variance in temperature preference can influence experimental conditions and outcomes.
• Ribonuclease H Activity: MMLV RT uniquely possesses inherent ribonuclease H (RNase H) activity, allowing it to degrade the RNA strand of an RNA:DNA hybrid molecule during cDNA synthesis. This feature aids in the removal of the RNA template post-synthesis, which can be advantageous in certain experimental contexts.
• Sensitivity to Inhibitors: AMV RT and MMLV RT exhibit disparate sensitivities to inhibitors or contaminants present in the reaction mixture. AMV RT tends to be more susceptible to inhibition, potentially affecting reaction efficiency and reliability.

In summary, while AMV RT and MMLV RT serve analogous functions in reverse transcription, their differences in thermostability, processivity, temperature optima, RNase H activity, and sensitivity to inhibitors underscore the need for thoughtful enzyme selection in molecular biology experiments. Researchers must weigh these distinctions against their experimental requirements to ensure optimal outcomes in cDNA synthesis and downstream applications.

Why Choose SBS Genetech's Mutant M-MLV Reverse Transcriptase?

SBS Genetech introduces Mutant M-MLV Reverse Transcriptase (Mutant M-MLV RT), a variant achieved through gene modification and recombinant technology. Compared to conventional M-MLV reverse transcriptase, this variant exhibits increased tolerance to inhibitors that impede cDNA synthesis, facilitating stable and specific cDNA synthesis from samples of diverse origins. Additionally, it accelerates reactions, reducing the incubation time from over 50 minutes to just 10 minutes. Furthermore, its capacity for continuous synthesis (yielding a final product exceeding 12 kb) has been significantly enhanced compared to previous iterations.

Unparalleled Cost-Effectiveness

With Mutant M-MLV Reverse Transcriptase priced as low as $340 for 1MU, it potentially stands as the most competitively priced M-MLV Reverse Transcriptase on the market.

We sincerely welcome you to experience the excellence of our products, supporting your research endeavors with cutting-edge technology and unparalleled quality. Whether you are engaged in gene expression studies or delving into viral genome analysis, our Mutant M-MLV Reverse Transcriptase will undoubtedly become an indispensable ally in your scientific journey.

At SBS Genetech, we prioritize not only competitive pricing but also the efficiency, specificity, and reliability of our products. Our mission is to provide researchers worldwide with the most advanced tools, empowering them to push the boundaries of science and unravel the mysteries of life.

Embark on your research journey with SBS Genetech's Mutant M-MLV Reverse Transcriptase, and let us together explore the boundless possibilities of science! Contact us today to unlock the full potential of your research endeavors.

Related: 

• Mutant M-MLV Reverse Transcriptase
• Thermo-stable M-MLV Reverse Transcriptase (RNase H-): active at 55°C
• Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-): active at 65°C
• Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-): active at 72°C
• TGI-RTase Reverse Transcriptase
• Warm Activated Reverse Transcriptase