In the fields of molecular biology and genetic engineering, restriction enzymes are a crucial class of enzymes widely employed for the modification and manipulation of DNA molecules. They have the ability to recognize specific DNA sequences and cleave them. This capability renders restriction enzymes indispensable in areas such as genetic engineering, DNA cloning, and DNA analysis. Among the numerous restriction enzymes, one stands out as the most commonly used: EcoRI.
EcoRI is a restriction endonuclease produced by Escherichia coli, commonly known as E. coli. Its name is derived from the initials of Escherichia coli ("E") and "coRI," indicating it was the first of the type R enzymes to be discovered. EcoRI was initially identified in 1970 by Israeli scientist Daniel Nathans and colleagues. The mechanism of action of this restriction enzyme involves recognizing and cleaving the specific six-base pair sequence GAATTC within DNA. The widespread application of EcoRI has made it a hallmark tool in molecular biology research.
EcoRI's status as one of the most commonly used restriction enzymes can be attributed to several key characteristics:
- High Specificity: EcoRI exclusively recognizes and cleaves the GAATTC six-base pair sequence within DNA, endowing it with high specificity to precisely locate target sequences within complex genomes.
- Reliability: EcoRI exhibits stable cleavage activity, ensuring reliable DNA digestion under various experimental conditions and guaranteeing the reproducibility and reliability of experimental results.
- Compatibility: EcoRI produces sticky ends upon cleavage, facilitating the convenient ligation of DNA fragments with complementary sticky ends, thus aiding in DNA fragment cloning and recombination.
- Commercial Availability: Due to its widespread use in research experiments, numerous suppliers offer high-purity EcoRI enzymes commercially, making it easily accessible to researchers for purchase and use.
In addition to EcoRI, there are many other commonly used restriction enzymes such as BamHI, HindIII, and NotI, each with specific recognition sequences and cleavage sites, widely employed for the modification and manipulation of DNA molecules. However, as one of the earliest discovered and most frequently used restriction enzymes, EcoRI has played an irreplaceable role in the field of molecular biology, serving as a vital tool in genetic engineering and DNA technology.
RapidCleave™ Fast Restriction Enzymes
Our company, SBS Genetech, proudly introduces the RapidCleave™ Fast Restriction Enzymes series, meticulously engineered to provide rapid cleavage of nucleic acids. Whether handling plasmid DNA, PCR products, or genomic DNA, RapidCleave™ offers astonishing speed and exceptional performance.
Features:
a. Enzyme Cleavage in 5~15 Minutes:
RapidCleave™ Fast Restriction Enzymes exhibit remarkable activity in both standard RapidCleave™ and RapidCleave™ Color Buffers. With cleavage completion possible in as little as 5 to 15 minutes, experimental efficiency is greatly enhanced.
b. Unparalleled One-Tube Experience:
Our dephosphorylation and ligation reagents demonstrate 100% activity within the RapidCleave™ Buffer, supporting one-tube reactions. Bid farewell to cumbersome steps and repetitive procedures, enjoying a smoother experimental journey of "Cleave - Modify - Ligase."
c. Unified Buffer Simplifies Multiple Digestions:
All enzymes in the RapidCleave™ series share a common restriction buffer called RapidCleave™ Buffer, significantly simplifying the digestion system and enabling convenient double or multiple enzyme digestions.
The RapidCleave™ Fast Restriction Enzymes series will become a reliable assistant in your laboratory, facilitating the achievement of research goals, accelerating scientific progress, and injecting new vitality into your work. Get in touch with us today to explore how RapidCleave™ can elevate your laboratory work and accelerate scientific discovery.