DNA-Free HS HiFi Taq DNA Polymerase, developed by SBS Genetech, is a cutting-edge enzyme designed to enhance the accuracy and reliability of PCR (Polymerase Chain Reaction) applications. This enzyme is specifically engineered to minimize the risk of contamination with bacterial and human DNA, making it an ideal choice for researchers and scientists who require high sensitivity and specificity in their experiments.
Key Features
- DNA-Free Certification: DNA-Free HS HiFi Taq DNA Polymerase is produced using a novel, closed single-use system combined with stringent quality-control testing. This process ensures that the enzyme has a certified low DNA contamination level, providing confidence in the qualitative and quantitative detection of low-abundance microorganisms.
- Hot-Start Technology: DNA-Free HS HiFi Taq DNA Polymerase is complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. This automatic hot start is activated after the initial denaturation step at 95°C for 5 minutes or at 98°C for 2 minutes, resulting in increased sensitivity, specificity, and yield while allowing convenient reaction assembly at room temperature.
- High-Fidelity PCR Amplification: DNA-Free HS HiFi Taq DNA Polymerase is a high-efficiency PCR enzyme developed based on the Barnes method. It combines KOD DNA polymerase, with strong 3'→5' exonuclease activity (proofreading activity), and Taq DNA polymerase in an optimal ratio, achieving superior PCR performance and improved fidelity.
- High Performance: DNA-Free HS HiFi Taq DNA Polymerase offers a high level of performance and lot-to-lot consistency. It has specially modified for enhanced amplification capability and extension speed, with fragment lengths up to 5-6 kb and an extension rate of 1-2 kb/min (72°C). The enzyme exhibits 5'→3' polymerase activity, weak 5'→3' exonuclease activity, and optimized 3'→5' exonuclease activity. A small amount of amplification product has 3'-dA overhangs.
Applications
DNA-Free HS HiFi Taq DNA Polymerase is suitable for a wide range of PCR-based applications, including:
- Amplification of DNA from Complex Genomic and Viral Templates: The enzyme's high sensitivity and specificity make it ideal for amplifying DNA from complex samples, ensuring accurate and reliable results.
- Hot-Start PCR: The built-in hot-start technology prevents nonspecific primer binding and the formation of primer dimers, improving the specificity of PCR results.
- RT-PCR: The enzyme is also suitable for reverse transcription PCR (RT-PCR), enabling the detection and quantification of RNA targets.
DNA-Free HS HiFi Taq DNA Polymerase represents a significant advancement in PCR technology. Its DNA-free certification, hot-start technology, and high performance make it an invaluable tool for researchers and scientists working in molecular biology, clinical diagnostics, and biotechnology. By minimizing the risk of contamination and enhancing the accuracy of PCR results, this enzyme is poised to revolutionize the field of DNA amplification.